Hotspot

rpoB-trnC-GCA

LSC · 26931-28104 · 1174 bp

Back to Mosla

Pi 0.00114
MarkerSeek score 45.20
Primer available yes
Label rpoB-trnC-GCA

Feature Metadata

label_name
rpoB-trnC-GCA
feature_type
igs
region
LSC
length_bp
1174
variable_sites
4
pi
0.00114
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
45.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoB-trnC-GCA_p1 GACAAACCCCGATCGATGAA CCGGATTTGAACTGGGGAAA 50.0 50.0 58.0 58.1 1326-1331 bp 1.0 79.9
rpoB-trnC-GCA_p2 GACAAACCCCGATCGATGAA TTTTCGTGATTCAGGCGACA 50.0 45.0 58.0 58.1 1347-1352 bp 1.0 79.5
rpoB-trnC-GCA_p3 GACATTGCCTCGTTTGCATC CCGGATTTGAACTGGGGAAA 50.0 50.0 58.1 58.1 1260-1265 bp 1.0 79.2
rpoB-trnC-GCA_p4 GACATTGCCTCGTTTGCATC TTTTCGTGATTCAGGCGACA 50.0 45.0 58.1 58.1 1281-1286 bp 1.0 78.8
rpoB-trnC-GCA_p5 TAGACATTGCCTCGTTTGCA CCGGATTTGAACTGGGGAAA 45.0 50.0 57.8 58.1 1262-1267 bp 1.0 78.6