Hotspot

rps16-trnQ-UUG

LSC · 5960-6990 · 1031 bp

Back to Mosla

Pi 0.00233
MarkerSeek score 52.50
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1031
variable_sites
6
pi
0.00233
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
52.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 GTCTTTCAAGTCGCACGTTG GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 1099-1122 bp 1.0 80.6
rps16-trnQ-UUG_p2 GTCTTTCAAGTCGCACGTTG TTAGACATCAACTTGGGGCG 50.0 50.0 58.0 57.9 1160-1183 bp 1.0 80.3
rps16-trnQ-UUG_p3 GTCTTTCAAGTCGCACGTTG GTCTCGCTATTCGGAGGTTC 50.0 55.0 58.0 57.9 1112-1135 bp 1.0 80.3
rps16-trnQ-UUG_p4 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 1092-1115 bp 1.0 79.6
rps16-trnQ-UUG_p5 AAGTCGCACGTTGCTTTCTA TTAGACATCAACTTGGGGCG 45.0 50.0 58.1 57.9 1153-1176 bp 1.0 79.3