Hotspot

ycf4-cemA

LSC · 63860-64813 · 954 bp

Back to Morus

Pi 0.00112
MarkerSeek score 48.70
Primer available yes
Label ycf4-cemA

Feature Metadata

label_name
ycf4-cemA
feature_type
igs
region
LSC
length_bp
954
variable_sites
4
pi
0.00112
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
48.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf4-cemA_p1 CAGAAAGCTGCTGAATTGGC AGAGAGATCCACCAAGGCAA 50.0 50.0 58.0 58.3 1081-1084 bp 1.0 77.6
ycf4-cemA_p2 CATTCCCTTGACTCGTACCG AGAGAGATCCACCAAGGCAA 55.0 50.0 58.1 58.3 1130-1133 bp 1.0 77.0
ycf4-cemA_p3 TTGGCCTATTTCTTGCGTGT AGAGAGATCCACCAAGGCAA 45.0 50.0 58.1 58.3 1066-1069 bp 1.0 76.9
ycf4-cemA_p4 ATTCCCTTGACTCGTACCGA AGAGAGATCCACCAAGGCAA 50.0 50.0 58.2 58.3 1129-1132 bp 1.0 76.4
ycf4-cemA_p5 TTCCCTTGACTCGTACCGAT AGAGAGATCCACCAAGGCAA 50.0 50.0 58.2 58.3 1128-1131 bp 1.0 76.4