Hotspot

trnL-UAA-trnF-GAA

LSC · 51127-51500 · 374 bp

Back to Morus

Pi 0.0433
MarkerSeek score 75.10
Primer available yes
Label trnL-UAA-trnF-GAA

Feature Metadata

label_name
trnL-UAA-trnF-GAA
feature_type
igs
region
LSC
length_bp
374
variable_sites
81
pi
0.0433
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
75.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-UAA-trnF-GAA_p1 AATCGTGAGGGTTCAAGTCC TCTACCAGCTGAGCTATCCC 50.0 55.0 57.5 58.0 423-432 bp 1.0 88.2
trnL-UAA-trnF-GAA_p2 AATCGTGAGGGTTCAAGTCC CAAATTGCTCATGTGCCAGG 50.0 50.0 57.5 58.0 486-495 bp 1.0 88.2
trnL-UAA-trnF-GAA_p3 AATCGTGAGGGTTCAAGTCC TTTGAACTGGTGACACGAGG 50.0 50.0 57.5 58.1 459-468 bp 1.0 87.8
trnL-UAA-trnF-GAA_p4 AATCGTGAGGGTTCAAGTCC AAATTGCTCATGTGCCAGGA 50.0 45.0 57.5 58.1 485-494 bp 1.0 87.6
trnL-UAA-trnF-GAA_p5 AATCGTGAGGGTTCAAGTCC AATTGCTCATGTGCCAGGAA 50.0 45.0 57.5 58.1 484-493 bp 1.0 87.6