Hotspot

psbZ-trnG-GCC

LSC · 38064-38879 · 816 bp

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Pi 0.0117
MarkerSeek score 65.80
Primer available yes
Label psbZ-trnG-GCC

Feature Metadata

label_name
psbZ-trnG-GCC
feature_type
igs
region
LSC
length_bp
816
variable_sites
42
pi
0.0117
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.99
markerseek_score
65.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbZ-trnG-GCC_p1 TTGCTTCTCCTGATGGTTGG AATCGAACCCGCATCTTCTC 50.0 50.0 57.8 58.1 594-984 bp 1.0 85.9
psbZ-trnG-GCC_p2 TTGCTTCTCCTGATGGTTGG GAATCGAACCCGCATCTTCT 50.0 50.0 57.8 58.1 595-985 bp 1.0 85.9
psbZ-trnG-GCC_p3 GCTTCTCCTGATGGTTGGTT AATCGAACCCGCATCTTCTC 50.0 50.0 57.8 58.1 592-982 bp 1.0 85.9
psbZ-trnG-GCC_p4 GCTTCTCCTGATGGTTGGTT GAATCGAACCCGCATCTTCT 50.0 50.0 57.8 58.1 593-983 bp 1.0 85.9
psbZ-trnG-GCC_p5 TCCTGATGGTTGGTTGGGTA AATCGAACCCGCATCTTCTC 50.0 50.0 58.3 58.1 587-977 bp 1.0 85.5