Hotspot

rps16-trnQ-UUG

LSC · 6650-7631 · 982 bp

Back to Morus

Pi 0.00111
MarkerSeek score 50.70
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
982
variable_sites
3
pi
0.00111
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
50.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 ACGTTGCCTTCTACCACATC GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.9 58.1 1028-1048 bp 1.0 80.4
rps16-trnQ-UUG_p2 GTTGCCTTCTACCACATCGT GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.9 58.1 1026-1046 bp 1.0 80.4
rps16-trnQ-UUG_p3 CACGTTGCCTTCTACCACAT GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.2 58.1 1029-1049 bp 1.0 79.6
rps16-trnQ-UUG_p4 CGGATCGTGTCCTTCAAGTC GAGGTTCGAATCCTTCCGTC 55.0 55.0 58.4 58.1 1050-1070 bp 1.0 78.2
rps16-trnQ-UUG_p5 GGATCGTGTCCTTCAAGTCG GAGGTTCGAATCCTTCCGTC 55.0 55.0 58.4 58.1 1049-1069 bp 1.0 78.2