Hotspot

rps2

LSC · 16216-16926 · 711 bp

Back to Moringa

Pi 0

MarkerSeek score 40.00

Primer available yes

Label rps2

Feature Metadata

label_name: rps2
feature_type: gene
region: LSC
length_bp: 711
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps2_p1	TTCTTTTGTGCGGTTCAGGA	GCCAATCTCCGGTAGAAGAG	45.0	55.0	58.0	57.5	902 bp	1.0	75.5
rps2_p2	TGTGCGGTTCAGGAGTAGTA	GCCAATCTCCGGTAGAAGAG	50.0	55.0	58.1	57.5	896 bp	1.0	75.2
rps2_p3	TTTGTGCGGTTCAGGAGTAG	GCCAATCTCCGGTAGAAGAG	50.0	55.0	57.8	57.5	898 bp	1.0	74.5
rps2_p4	GCGGTTCAGGAGTAGTAACC	GCCAATCTCCGGTAGAAGAG	55.0	55.0	57.7	57.5	893 bp	1.0	73.5
rps2_p5	TCTTTTGTGCGGTTCAGGAG	GCCAATCTCCGGTAGAAGAG	50.0	55.0	58.4	57.5	901 bp	1.0	72.6