Hotspot

rps16

LSC · 5164-6329 · 1166 bp

Back to Moringa

Pi 0

MarkerSeek score 40.00

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1166
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	TGAGTCATCCACACCCTCTT	TGTACCGATCAAACAATGACT	50.0	38.1	58.0	55.3	1276 bp	1.0	49.8
rps16_p2	ATGAGTCATCCACACCCTCT	TGTACCGATCAAACAATGACT	50.0	38.1	57.7	55.3	1277 bp	1.0	48.0
rps16_p3	TGAGTCATCCACACCCTCTT	ATGTACCGATCAAACAATGACT	50.0	36.4	58.0	55.8	1277 bp	1.0	46.3
rps16_p4	TGAGTCATCCACACCCTCTT	TGTACCGATCAAACAATGAC	50.0	40.0	58.0	53.7	1276 bp	1.0	45.0
rps16_p5	ATGAGTCATCCACACCCTCT	ATGTACCGATCAAACAATGACT	50.0	36.4	57.7	55.8	1278 bp	1.0	44.4