Hotspot

psbA

LSC · 435-1496 · 1062 bp

Back to Moringa

Pi 0

MarkerSeek score 40.00

Primer available yes

Label psbA

Feature Metadata

label_name: psbA
feature_type: gene
region: LSC
length_bp: 1062
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	AGGAGCAATACCAACCTTCT	ATTTGTGTGCTTGGGAGTCC	45.0	50.0	56.1	58.4	1203 bp	1.0	61.9
psbA_p2	AAGGAGCAATACCAACCTTCT	ATTTGTGTGCTTGGGAGTCC	42.9	50.0	56.9	58.4	1204 bp	1.0	60.2
psbA_p3	AGGAGCAATACCAACCTTCTT	ATTTGTGTGCTTGGGAGTCC	42.9	50.0	56.9	58.4	1203 bp	1.0	60.2
psbA_p4	GGAGCAATACCAACCTTCTTG	ATTTGTGTGCTTGGGAGTCC	47.6	50.0	56.9	58.4	1202 bp	1.0	59.9
psbA_p5	AGGAGCAATACCAACCTTCTTG	ATTTGTGTGCTTGGGAGTCC	45.5	50.0	58.3	58.4	1203 bp	1.0	58.5