Hotspot

atpF

LSC · 12000-13290 · 1291 bp

Back to Moringa

Pi 0

MarkerSeek score 40.00

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: LSC
length_bp: 1291
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	CCGTTTCGGCTACCACAATA	CCTGAGGCGGAAGGAAAAAT	50.0	50.0	58.0	58.2	2709 bp	1.0	78.6
atpF_p2	CCGTTTCGGCTACCACAATA	GTATCGCGAGACAACCTGAG	50.0	55.0	58.0	58.2	2723 bp	1.0	78.5
atpF_p3	CCGTTTCGGCTACCACAATA	GGAGTTGGTCAAGGTACTGC	50.0	55.0	58.0	58.2	2763 bp	1.0	78.4
atpF_p4	CCCTTTCCTGGAAAGTGGTC	CCTGAGGCGGAAGGAAAAAT	55.0	50.0	58.1	58.2	2673 bp	1.0	78.0
atpF_p5	GTCGCCTACTTGAAGTACGG	CCTGAGGCGGAAGGAAAAAT	55.0	50.0	58.1	58.2	2135 bp	1.0	78.0