Hotspot

trnK-UUU-rps16

LSC · 4305-5163 · 859 bp

Back to Moringa

Pi 0
MarkerSeek score 40.00
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
859
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AACTCTACCGCTGGTATGGA AAAAAGGCGCTCAACCTACA 50.0 45.0 57.8 58.0 1056 bp 1.0 78.6
trnK-UUU-rps16_p2 AAAGCCGAGTACTCTACCGT AAAAAGGCGCTCAACCTACA 50.0 45.0 58.2 58.0 988 bp 1.0 78.5
trnK-UUU-rps16_p3 AAGCCGAGTACTCTACCGTT AAAAAGGCGCTCAACCTACA 50.0 45.0 58.2 58.0 987 bp 1.0 78.5
trnK-UUU-rps16_p4 AACTCTACCGCTGGTATGGA AGAAGGCAGGGGTTTTTACG 50.0 50.0 57.8 58.1 1014 bp 1.0 78.0
trnK-UUU-rps16_p5 AAGCCGAGTACTCTACCGTT AGAAGGCAGGGGTTTTTACG 50.0 50.0 58.2 58.1 945 bp 1.0 77.9