Hotspot

rps18

LSC · 73216-73855 · 640 bp

Back to Morina

Pi 0.0872
MarkerSeek score 80.10
Primer available yes
Label rps18

Feature Metadata

label_name
rps18
feature_type
gene
region
LSC
length_bp
640
variable_sites
90
pi
0.0872
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.86
markerseek_score
80.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps18_p1 TAGATCGAACCATGCGTGTG GAGTTCCCCGGAGAATGAAC 50.0 55.0 58.1 58.0 903-1098 bp 1.0 91.2
rps18_p2 TAGATCGAACCATGCGTGTG GTTCCCCGGAGAATGAACTC 50.0 55.0 58.1 58.0 901-1096 bp 1.0 91.2
rps18_p3 TAGATCGAACCATGCGTGTG TCTTCCCTTCCCCGATTCTT 50.0 50.0 58.1 58.0 784-950 bp 0.285714 54.6
rps18_p4 TAGATCGAACCATGCGTGTG TCTTCTTCCCTTCCCCGATT 50.0 50.0 58.1 58.0 787-953 bp 0.285714 54.6
rps18_p5 TAGATCGAACCATGCGTGTG TTCTTCCCTTCCCCGATTCT 50.0 50.0 58.1 58.0 785-951 bp 0.285714 54.6