Hotspot

accD

LSC · 60817-63021 · 2205 bp

Back to Morina

Pi 0.0655
MarkerSeek score 71.10
Primer available yes
Label accD

Feature Metadata

label_name
accD
feature_type
gene
region
LSC
length_bp
2205
variable_sites
260
pi
0.0655
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.80
markerseek_score
71.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
accD_p1 TGTGAGGGGTGGACAGTTAT TACGACCCAACAGGGATTCT 50.0 50.0 58.0 58.1 2638 bp 0.142857 45.0
accD_p2 CATGTCCACTCTTGTGAGGG TACGACCCAACAGGGATTCT 55.0 50.0 57.9 58.1 2650 bp 0.142857 44.5
accD_p3 TTGTGAGGGGTGGACAGTTA TACGACCCAACAGGGATTCT 50.0 50.0 58.2 58.1 2639 bp 0.142857 43.8
accD_p4 AGCATGTCCACTCTTGTGAG TACGACCCAACAGGGATTCT 50.0 50.0 57.5 58.1 2652 bp 0.142857 41.5
accD_p5 GCATGTCCACTCTTGTGAGG TACGACCCAACAGGGATTCT 55.0 50.0 58.6 58.1 2651 bp 0.142857 40.9