Hotspot

trnL-CAA-ycf2

IRa · 149381-150084 · 704 bp

Back to Morina

Pi 0.124
MarkerSeek score 66.40
Primer available yes
Label trnL-CAA-ycf2

Feature Metadata

label_name
trnL-CAA-ycf2
feature_type
igs
region
IRa
length_bp
704
variable_sites
70
pi
0.124
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.35
markerseek_score
66.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-CAA-ycf2_p1 AAATGGTAGACACGCGAGAC CCCATTCCTTAGCCGGAAAG 50.0 55.0 58.0 58.3 361-828 bp 1.0 89.4
trnL-CAA-ycf2_p2 TGAAATGGTAGACACGCGAG CCCATTCCTTAGCCGGAAAG 50.0 55.0 58.0 58.3 363-830 bp 1.0 89.4
trnL-CAA-ycf2_p3 AAATGGTAGACACGCGAGAC GCCGGAAAGATATGTGACCA 50.0 50.0 58.0 57.7 350-817 bp 1.0 89.3
trnL-CAA-ycf2_p4 TGAAATGGTAGACACGCGAG GCCGGAAAGATATGTGACCA 50.0 50.0 58.0 57.7 352-819 bp 1.0 89.3
trnL-CAA-ycf2_p5 AAATGGTAGACACGCGAGAC ATTTCCCATTCCTTAGCCGG 50.0 50.0 58.0 57.6 365-832 bp 1.0 89.0