Hotspot

trnN-GUU-trnR-ACG

IRa · 134287-135064 · 778 bp

Back to Morina

Pi 0.0819
MarkerSeek score 76.20
Primer available yes
Label trnN-GUU-trnR-ACG

Feature Metadata

label_name
trnN-GUU-trnR-ACG
feature_type
igs
region
IRa
length_bp
778
variable_sites
126
pi
0.0819
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.66
markerseek_score
76.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnN-GUU-trnR-ACG_p1 TGGTCGTAGGTTCGAATCCT CCTTGTTCCTACATGACCCG 50.0 55.0 58.2 58.0 1288-1531 bp 0.857143 84.8
trnN-GUU-trnR-ACG_p2 TGGTCGTAGGTTCGAATCCT AAAAGCTCTGTCCTCCCTCT 50.0 50.0 58.2 58.0 1203-1446 bp 0.857143 84.7
trnN-GUU-trnR-ACG_p3 AAGTTCCGTCGTTGAGTGAC CCTTGTTCCTACATGACCCG 50.0 55.0 58.2 58.0 1802 bp 0.142857 44.3
trnN-GUU-trnR-ACG_p4 AAGTTCCGTCGTTGAGTGAC AAAAGCTCTGTCCTCCCTCT 50.0 50.0 58.2 58.0 1717 bp 0.142857 44.2
trnN-GUU-trnR-ACG_p5 TGGAAAGTTACAATCCCCGC CCTTGTTCCTACATGACCCG 50.0 55.0 58.2 58.0 1768 bp 0.142857 44.2