Hotspot

rpl32-trnL-UAG

SSC · 117856-118684 · 829 bp

Back to Morina

Pi 0.0335
MarkerSeek score 68.60
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
829
variable_sites
53
pi
0.0335
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.86
markerseek_score
68.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GAGGATTGGCGGAATTCACT AGCGTGTCTACCGATTTCAC 50.0 50.0 58.0 58.0 1279-1348 bp 1.0 88.4
rpl32-trnL-UAG_p2 AGAGGATTGGCGGAATTCAC TGGAGGGCGGGTACTAATAG 50.0 55.0 58.0 58.0 1823-1892 bp 1.0 87.9
rpl32-trnL-UAG_p3 GAGGATTGGCGGAATTCACT TGGAGGGCGGGTACTAATAG 50.0 55.0 58.0 58.0 1822-1891 bp 1.0 87.9
rpl32-trnL-UAG_p4 AGAGGATTGGCGGAATTCAC GGAGGGCGGGTACTAATAGT 50.0 55.0 58.0 58.0 1822-1891 bp 1.0 87.9
rpl32-trnL-UAG_p5 GAGGATTGGCGGAATTCACT GGAGGGCGGGTACTAATAGT 50.0 55.0 58.0 58.0 1821-1890 bp 1.0 87.9