Hotspot

trnN-GUU-ndhF

SSC · 114536-115324 · 789 bp

Back to Morina

Pi 0.157
MarkerSeek score 73.70
Primer available yes
Label trnN-GUU-ndhF

Feature Metadata

label_name
trnN-GUU-ndhF
feature_type
igs
region
SSC
length_bp
789
variable_sites
321
pi
0.157
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.99
markerseek_score
73.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnN-GUU-ndhF_p1 AACAAAGGATTAGCCCGGAC ACAGGTCGTGTGAACCAAAA 50.0 45.0 57.9 57.9 1560-1749 bp 0.285714 61.5
trnN-GUU-ndhF_p2 CCCGTAACTCTTCTTCAGCC AACAGGTCGTGTGAACCAAA 55.0 45.0 58.0 57.9 1705 bp 0.142857 44.7
trnN-GUU-ndhF_p3 CCCGTAACTCTTCTTCAGCC ACAGGTCGTGTGAACCAAAA 55.0 45.0 58.0 57.9 1704 bp 0.142857 44.7
trnN-GUU-ndhF_p4 TGGAACAAAGGATTAGCCCG AACAGGTCGTGTGAACCAAA 50.0 45.0 57.9 57.9 1564 bp 0.142857 43.8
trnN-GUU-ndhF_p5 TGGAACAAAGGATTAGCCCG ACAGGTCGTGTGAACCAAAA 50.0 45.0 57.9 57.9 1563 bp 0.142857 43.8