Hotspot

ycf2-trnL-CAA

IRb · 98666-99369 · 704 bp

Back to Morina

Pi 0.127
MarkerSeek score 66.60
Primer available yes
Label ycf2-trnL-CAA

Feature Metadata

label_name
ycf2-trnL-CAA
feature_type
igs
region
IRb
length_bp
704
variable_sites
71
pi
0.127
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.37
markerseek_score
66.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf2-trnL-CAA_p1 CCCATTCCTTAGCCGGAAAG AAATGGTAGACACGCGAGAC 55.0 50.0 58.3 58.0 361-828 bp 1.0 89.4
ycf2-trnL-CAA_p2 CCCATTCCTTAGCCGGAAAG TGAAATGGTAGACACGCGAG 55.0 50.0 58.3 58.0 363-830 bp 1.0 89.4
ycf2-trnL-CAA_p3 GCCGGAAAGATATGTGACCA AAATGGTAGACACGCGAGAC 50.0 50.0 57.7 58.0 350-817 bp 1.0 89.3
ycf2-trnL-CAA_p4 GCCGGAAAGATATGTGACCA TGAAATGGTAGACACGCGAG 50.0 50.0 57.7 58.0 352-819 bp 1.0 89.3
ycf2-trnL-CAA_p5 ATTTCCCATTCCTTAGCCGG AAATGGTAGACACGCGAGAC 50.0 50.0 57.6 58.0 365-832 bp 1.0 89.0