Hotspot

ndhC-trnV-UAC

LSC · 53571-54612 · 1042 bp

Back to Morina

Pi 0.0787
MarkerSeek score 82.20
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1042
variable_sites
234
pi
0.0787
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.88
markerseek_score
82.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 ACCATTCCAATGCCCCTTTT CCGAATCAGGTGCCCATTTA 45.0 50.0 58.0 57.9 1795-1888 bp 1.0 91.2
ndhC-trnV-UAC_p2 TTTTCCGGCCCTTTGCTAAT CCGAATCAGGTGCCCATTTA 45.0 50.0 58.1 57.9 1556-1649 bp 1.0 91.0
ndhC-trnV-UAC_p3 TGGTTCAGGCATTTGCTCAT CCGAATCAGGTGCCCATTTA 45.0 50.0 58.1 57.9 2154-2246 bp 1.0 91.0
ndhC-trnV-UAC_p4 ACCATTCCAATGCCCCTTTT AGCCTGACAAATGGTTTGGT 45.0 45.0 58.0 57.9 1815-1908 bp 1.0 91.0
ndhC-trnV-UAC_p5 AGAAAGTTTTTCCGGCCCTT CCGAATCAGGTGCCCATTTA 45.0 50.0 57.9 57.9 1563-1656 bp 1.0 90.9