Hotspot

ycf3-trnS-GGA

LSC · 47681-47995 · 315 bp

Back to Morina

Pi 0.0895
MarkerSeek score 72.80
Primer available yes
Label ycf3-trnS-GGA

Feature Metadata

label_name
ycf3-trnS-GGA
feature_type
igs
region
LSC
length_bp
315
variable_sites
60
pi
0.0895
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.97
markerseek_score
72.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf3-trnS-GGA_p1 CCCTATGCCCCCTATTGGTA GGAGCGCGTCAACTAGTTAA 55.0 50.0 57.9 58.0 1394-1866 bp 1.0 91.5
ycf3-trnS-GGA_p2 GCATCTGAGGCTGCACTAAT GGAGCGCGTCAACTAGTTAA 50.0 50.0 58.0 58.0 1282-1486 bp 0.285714 56.3
ycf3-trnS-GGA_p3 TACCCTCAGTAAGCGTCGAT GGAGCGCGTCAACTAGTTAA 50.0 50.0 58.0 58.0 1225-1429 bp 0.285714 56.2
ycf3-trnS-GGA_p4 CTGAGGCTGCACTAATCAGG GGAGCGCGTCAACTAGTTAA 55.0 50.0 58.0 58.0 1278-1482 bp 0.285714 56.1
ycf3-trnS-GGA_p5 GCATCTGAGGCTGCACTAAT TATGGCGCGGGATAAACAAA 50.0 45.0 58.0 58.0 1190-1394 bp 0.285714 56.1