Hotspot

trnS-GCU-trnG-GCC

LSC · 9762-10536 · 775 bp

Back to Morina

Pi 0.0371
MarkerSeek score 72.50
Primer available yes
Label trnS-GCU-trnG-GCC

Feature Metadata

label_name
trnS-GCU-trnG-GCC
feature_type
igs
region
LSC
length_bp
775
variable_sites
57
pi
0.0371
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.97
markerseek_score
72.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-GCC_p1 ATTAGCAATCCGCCGCTTTA AGGGGCTTAATAGAACGAATCA 45.0 40.9 58.0 57.0 834-861 bp 0.285714 29.4
trnS-GCU-trnG-GCC_p2 ATTAGCAATCCGCCGCTTTA GGGGCTTAATAGAACGAATCAC 45.0 45.5 58.0 56.9 833-860 bp 0.285714 29.2
trnS-GCU-trnG-GCC_p3 GCTCGTACAACGGATTAGCA AGGGGCTTAATAGAACGAATCA 50.0 40.9 58.1 57.0 847-874 bp 0.285714 29.0
trnS-GCU-trnG-GCC_p4 GCTCGTACAACGGATTAGCA GGGGCTTAATAGAACGAATCAC 50.0 45.5 58.1 56.9 846-873 bp 0.285714 28.8
trnS-GCU-trnG-GCC_p5 ACGGAAAGAGAGGGATTCGA AGGGGCTTAATAGAACGAATCA 50.0 40.9 58.2 57.0 883-910 bp 0.285714 28.4