Hotspot

trnH-GUG-psbA

LSC · 518-874 · 357 bp

Back to Morina

Pi 0.117
MarkerSeek score 69.80
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
357
variable_sites
40
pi
0.117
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.46
markerseek_score
69.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 CAAGAGAAGTATGTAGATTACTAAA CGGATGTAGCCAAGTGGATC 28.0 55.0 52.2 58.1 199 bp 0.142857 5.7
trnH-GUG-psbA_p2 CAAGAGAAGTATGTAGATTACTAAA CTTCTCTTGGGCGGATGTAG 28.0 55.0 52.2 57.8 210 bp 0.142857 5.7
trnH-GUG-psbA_p3 CAAGAGAAGTATGTAGATTACTAAA ACATACTTCTCTTGGGCGGA 28.0 50.0 52.2 58.4 215 bp 0.142857 5.7
trnH-GUG-psbA_p4 CAAGAGAAGTATGTAGATTACTAAA ACTTCTCTTGGGCGGATGTA 28.0 50.0 52.2 58.4 211 bp 0.142857 5.7
trnH-GUG-psbA_p5 CAAGAGAAGTATGTAGATTACTAAA TACTTCTCTTGGGCGGATGT 28.0 50.0 52.2 58.4 212 bp 0.142857 5.7