Hotspot

ycf1

SSC · 128353-134007 · 5655 bp

Back to Morella

Pi 0.00687

MarkerSeek score 42.50

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5655
variable_sites: 71
pi: 0.00687
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 42.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GATCGACTTGGTACGCGAAT	CGAGCATTTTGTTGGAACCC	50.0	50.0	58.2	57.9	1040 bp	0.75	69.3
ycf1_p2	ATCAATATCGACCCAGGCCT	CGAGCATTTTGTTGGAACCC	50.0	50.0	58.3	57.9	613 bp	0.75	68.5
ycf1_p3	TGGTTGGTTTGAGAGAGCTG	CGAGCATTTTGTTGGAACCC	50.0	50.0	57.7	57.9	1066 bp	0.75	68.4
ycf1_p4	ATCGACTTGGTACGCGAATT	CGAGCATTTTGTTGGAACCC	45.0	50.0	57.7	57.9	1039 bp	0.75	68.2
ycf1_p5	TCCTGTTGGTGACGGTATCA	CGAGCATTTTGTTGGAACCC	50.0	50.0	58.4	57.9	629 bp	0.75	67.8