Hotspot

psbB

LSC · 76152-77678 · 1527 bp

Back to Morella

Pi 0.00142

MarkerSeek score 59.70

Primer available yes

Label psbB

Feature Metadata

label_name: psbB
feature_type: gene
region: LSC
length_bp: 1527
variable_sites: 4
pi: 0.00142
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbB_p1	CCACTGAAATGGGGAGGTTT	TCAAGATTTATCCGGTACCCT	50.0	42.9	57.7	55.8	1701-1702 bp	1.0	52.5
psbB_p2	ATCCACTGAAATGGGGAGGT	TCAAGATTTATCCGGTACCCT	50.0	42.9	58.3	55.8	1703-1704 bp	1.0	52.5
psbB_p3	TCCACTGAAATGGGGAGGTT	TCAAGATTTATCCGGTACCCT	50.0	42.9	58.5	55.8	1702-1703 bp	1.0	50.6
psbB_p4	CCACTGAAATGGGGAGGTTT	ATCAAGATTTATCCGGTACCCT	50.0	40.9	57.7	56.4	1702-1703 bp	1.0	48.9
psbB_p5	ATCCACTGAAATGGGGAGGT	ATCAAGATTTATCCGGTACCCT	50.0	40.9	58.3	56.4	1704-1705 bp	1.0	48.8