Hotspot

rpl32-trnL-UAG

SSC · 117635-118607 · 973 bp

Back to Morella

Pi 0.029

MarkerSeek score 65.30

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 973
variable_sites: 46
pi: 0.029
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 65.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	ACCCAAGAAAATCAGAGCTGT	AGCGTGTCTACCGATTTCAC	42.9	50.0	57.5	58.0	1658-1802 bp	1.0	71.5
rpl32-trnL-UAG_p2	ACCCAAGAAAATCAGAGCTGT	CAGCGTGTCTACCGATTTCA	42.9	50.0	57.5	58.0	1659-1803 bp	1.0	71.5
rpl32-trnL-UAG_p3	ACCCAAGAAAATCAGAGCTGT	ATAACACTTGGCGCGGTTAT	42.9	45.0	57.5	58.0	2133-2281 bp	1.0	70.8
rpl32-trnL-UAG_p4	ACCCAAGAAAATCAGAGCTGT	AAAAATAACACTTGGCGCGG	42.9	45.0	57.5	57.9	2137-2285 bp	1.0	70.6
rpl32-trnL-UAG_p5	ACCCAAGAAAATCAGAGCTGT	ATGAGATTGGAGAGCGGGTA	42.9	50.0	57.5	57.9	2215-2363 bp	1.0	70.4