Hotspot

ndhF-rpl32

SSC · 116536-117469 · 934 bp

Back to Morella

Pi 0.0141
MarkerSeek score 58.10
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
934
variable_sites
23
pi
0.0141
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
58.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 AAGTCCCACGCCTATTAACA TTTGATATAGAAGTGCGTTTTT 45.0 27.3 56.5 52.4 1047-1062 bp 1.0 41.9
ndhF-rpl32_p2 AAGTCCCACGCCTATTAACA TTTTGATATAGAAGTGCGTTTT 45.0 27.3 56.5 52.4 1048-1063 bp 1.0 41.9
ndhF-rpl32_p3 AAGTCCCACGCCTATTAACA TTTTTGATATAGAAGTGCGTTT 45.0 27.3 56.5 52.4 1049-1064 bp 1.0 41.9
ndhF-rpl32_p4 AAGTCCCACGCCTATTAACA TTTTGATATAGAAGTGCGTTTTT 45.0 26.1 56.5 53.3 1048-1063 bp 1.0 41.9
ndhF-rpl32_p5 AAGTCCCACGCCTATTAACA TTTTTGATATAGAAGTGCGTTTT 45.0 26.1 56.5 53.3 1049-1064 bp 1.0 41.9