Hotspot

psaJ-rpl33

LSC · 70764-71253 · 490 bp

Back to Morella

Pi 0.00648

MarkerSeek score 63.70

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 490
variable_sites: 6
pi: 0.00648
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	TGGCACCGGTAGTAAGTACT	TAGCACCATTTCGAGCACAA	50.0	45.0	57.8	57.8	650-661 bp	1.0	79.0
psaJ-rpl33_p2	TGGCACCGGTAGTAAGTACT	TTAGCACCATTTCGAGCACA	50.0	45.0	57.8	57.8	651-662 bp	1.0	79.0
psaJ-rpl33_p3	GTTCGGGTCTTTAGCAGGTC	TAGCACCATTTCGAGCACAA	55.0	45.0	58.3	57.8	625-636 bp	1.0	78.6
psaJ-rpl33_p4	GTTCGGGTCTTTAGCAGGTC	TTAGCACCATTTCGAGCACA	55.0	45.0	58.3	57.8	626-637 bp	1.0	78.6
psaJ-rpl33_p5	GTGGCACCGGTAGTAAGTAC	TAGCACCATTTCGAGCACAA	55.0	45.0	57.7	57.8	651-662 bp	1.0	78.5