Hotspot

accD-psaI

LSC · 62295-63141 · 847 bp

Back to Morella

Pi 0.00571

MarkerSeek score 62.60

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 847
variable_sites: 9
pi: 0.00571
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	GATCCAATCGTACCGCGTAA	CCATTGCAATTGCCGGAAAT	50.0	45.0	57.9	58.0	1007-1013 bp	1.0	79.5
accD-psaI_p2	TCCAATCGTACCGCGTAATC	CCATTGCAATTGCCGGAAAT	50.0	45.0	57.9	58.0	1005-1011 bp	1.0	79.5
accD-psaI_p3	CCAATCGTACCGCGTAATCT	CCATTGCAATTGCCGGAAAT	50.0	45.0	57.9	58.0	1004-1010 bp	1.0	79.5
accD-psaI_p4	GATCCAATCGTACCGCGTAA	AAGAAGCCATTGCAATTGCC	50.0	45.0	57.9	57.9	1013-1019 bp	1.0	78.9
accD-psaI_p5	TCCAATCGTACCGCGTAATC	AAGAAGCCATTGCAATTGCC	50.0	45.0	57.9	57.9	1011-1017 bp	1.0	78.9