Hotspot

trnT-GGU-psbD

LSC · 33856-35373 · 1518 bp

Back to Morella

Pi 0.00801

MarkerSeek score 58.70

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1518
variable_sites: 22
pi: 0.00801
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GTGGTAGAGTAACGCCATGG	ACAAAAACGAAACGGTCCCT	55.0	45.0	58.1	58.2	1668-1678 bp	1.0	78.7
trnT-GGU-psbD_p2	GTGGTAGAGTAACGCCATGG	GACCGGACCAACCTACAAAA	55.0	50.0	58.1	57.7	1682-1692 bp	1.0	78.6
trnT-GGU-psbD_p3	TCAGTGGTAGAGTAACGCCA	ACAAAAACGAAACGGTCCCT	50.0	45.0	58.1	58.2	1671-1681 bp	1.0	78.5
trnT-GGU-psbD_p4	GCTCAGTGGTAGAGTAACGC	ACAAAAACGAAACGGTCCCT	55.0	45.0	58.1	58.2	1673-1683 bp	1.0	78.5
trnT-GGU-psbD_p5	TCAGTGGTAGAGTAACGCCA	GACCGGACCAACCTACAAAA	50.0	50.0	58.1	57.7	1685-1695 bp	1.0	78.4