Hotspot

trnD-GUC-trnY-GUA

LSC · 32350-32831 · 482 bp

Back to Morella

Pi 0.00553
MarkerSeek score 61.90
Primer available yes
Label trnD-GUC-trnY-GUA

Feature Metadata

label_name
trnD-GUC-trnY-GUA
feature_type
igs
region
LSC
length_bp
482
variable_sites
5
pi
0.00553
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.98
markerseek_score
61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnD-GUC-trnY-GUA_p1 CGGTGCTCTGACCAATTGAA TGGAATTGATTCTGCCTGGG 50.0 50.0 58.5 57.9 606-613 bp 1.0 76.8
trnD-GUC-trnY-GUA_p2 CGGTGCTCTGACCAATTGAA GGAATTGATTCTGCCTGGGT 50.0 50.0 58.5 57.9 605-612 bp 1.0 76.8
trnD-GUC-trnY-GUA_p3 CGGTGCTCTGACCAATTGAA TGGCAATATGTCTACGCTGG 50.0 50.0 58.5 57.8 545-552 bp 1.0 76.3
trnD-GUC-trnY-GUA_p4 CGGTGCTCTGACCAATTGAA GGCAATATGTCTACGCTGGT 50.0 50.0 58.5 57.8 544-551 bp 1.0 76.3
trnD-GUC-trnY-GUA_p5 CGGTGCTCTGACCAATTGAA AATTGATTCTGCCTGGGTCG 50.0 50.0 58.5 58.2 603-610 bp 1.0 76.1