Hotspot

psbK-psbI

LSC · 8397-8794 · 398 bp

Back to Morella

Pi 0.00378

MarkerSeek score 62.40

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 398
variable_sites: 3
pi: 0.00378
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	GTTTGGCAAGCTGCTGTAAG	TCTTCACGTCCAGGATTACG	50.0	50.0	57.9	57.1	517-535 bp	1.0	75.3
psbK-psbI_p2	CTTTTGTTTGGCAAGCTGCT	TCTTCACGTCCAGGATTACG	45.0	50.0	58.1	57.1	522-540 bp	1.0	75.2
psbK-psbI_p3	TGGCAAGCTGCTGTAAGTTT	TCTTCACGTCCAGGATTACG	45.0	50.0	58.3	57.1	514-532 bp	1.0	73.2
psbK-psbI_p4	TTGGCAAGCTGCTGTAAGTT	TCTTCACGTCCAGGATTACG	45.0	50.0	58.3	57.1	515-533 bp	1.0	73.2
psbK-psbI_p5	TTTGGCAAGCTGCTGTAAGT	TCTTCACGTCCAGGATTACG	45.0	50.0	58.3	57.1	516-534 bp	1.0	73.2