Hotspot

rps16-trnQ-UUG

LSC · 6210-7761 · 1552 bp

Back to Morella

Pi 0.0106
MarkerSeek score 68.10
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1552
variable_sites
28
pi
0.0106
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.99
markerseek_score
68.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 ATCCTTCACCTTCAAGTCGC GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.9 58.1 1624-1648 bp 1.0 81.7
rps16-trnQ-UUG_p2 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 1611-1635 bp 1.0 81.4
rps16-trnQ-UUG_p3 TCCACAGACGACCATATCCT GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.5 58.1 1639-1663 bp 1.0 78.9
rps16-trnQ-UUG_p4 ATCCTTCACCTTCAAGTCGC GGGTTTTGGTCCCGGTATTC 50.0 55.0 57.9 58.5 1645-1669 bp 1.0 78.0
rps16-trnQ-UUG_p5 AAGTCGCACGTTGCTTTCTA GGGTTTTGGTCCCGGTATTC 45.0 55.0 58.1 58.5 1632-1656 bp 1.0 77.7