Hotspot

rpl16-rps3

LSC · 80612-80761 · 150 bp

Back to Moraea

Pi 0.04
MarkerSeek score 66.30
Primer available yes
Label rpl16-rps3

Feature Metadata

label_name
rpl16-rps3
feature_type
igs
region
LSC
length_bp
150
variable_sites
6
pi
0.04
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
66.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl16-rps3_p1 TCAGAGTGGGGTTCCATCTT TCGAACTATCTATGGAGTATTAGGC 50.0 40.0 58.0 57.4 298-304 bp 1.0 41.9
rpl16-rps3_p2 TTCAGAGTGGGGTTCCATCT TCGAACTATCTATGGAGTATTAGGC 50.0 40.0 58.0 57.4 299-305 bp 1.0 41.9
rpl16-rps3_p3 TCAGAGTGGGGTTCCATCTT CGAACTATCTATGGAGTATTAGGCT 50.0 40.0 58.0 57.4 297-303 bp 1.0 41.9
rpl16-rps3_p4 TTCAGAGTGGGGTTCCATCT CGAACTATCTATGGAGTATTAGGCT 50.0 40.0 58.0 57.4 298-304 bp 1.0 41.9
rpl16-rps3_p5 GTTCAGAGTGGGGTTCCATC TCGAACTATCTATGGAGTATTAGGC 55.0 40.0 57.9 57.4 300-306 bp 1.0 41.8