Hotspot

psbB-psbT

LSC · 71965-72144 · 180 bp

Back to Moraea

Pi 0.0294
MarkerSeek score 70.70
Primer available yes
Label psbB-psbT

Feature Metadata

label_name
psbB-psbT
feature_type
igs
region
LSC
length_bp
180
variable_sites
5
pi
0.0294
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.94
markerseek_score
70.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbB-psbT_p1 GGTGCTAGAACCCTGTTCAG AACCTTAGGTGGTTCTCGAA 55.0 45.0 57.9 56.1 379-387 bp 1.0 67.1
psbB-psbT_p2 GGTGCTAGAACCCTGTTCAG ACCTTAGGTGGTTCTCGAAA 55.0 45.0 57.9 56.1 378-386 bp 1.0 67.1
psbB-psbT_p3 GGTGCTAGAACCCTGTTCAG AACCTTAGGTGGTTCTCGAAA 55.0 42.9 57.9 56.9 379-387 bp 1.0 65.3
psbB-psbT_p4 GGTGCTAGAACCCTGTTCAG ACCTTAGGTGGTTCTCGAAAA 55.0 42.9 57.9 56.9 378-386 bp 1.0 65.3
psbB-psbT_p5 GGTGCTAGAACCCTGTTCAG ACCTTAGGTGGTTCTCGAAAAA 55.0 40.9 57.9 57.6 378-386 bp 1.0 62.9