Hotspot

petL-petG

LSC · 64119-64302 · 184 bp

Back to Moraea

Pi 0.0333
MarkerSeek score 70.60
Primer available yes
Label petL-petG

Feature Metadata

label_name
petL-petG
feature_type
igs
region
LSC
length_bp
184
variable_sites
6
pi
0.0333
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
70.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petL-petG_p1 AACCCCAGCTCTGTTGATTG GATCACCGCGTCTGTATTGT 50.0 50.0 58.1 58.1 330-333 bp 1.0 81.1
petL-petG_p2 CCCAGCTCTGTTGATTGGTT GATCACCGCGTCTGTATTGT 50.0 50.0 58.1 58.1 327-330 bp 1.0 81.1
petL-petG_p3 AACCCCAGCTCTGTTGATTG TGATCACCGCGTCTGTATTG 50.0 50.0 58.1 58.1 331-334 bp 1.0 81.1
petL-petG_p4 CCCAGCTCTGTTGATTGGTT TGATCACCGCGTCTGTATTG 50.0 50.0 58.1 58.1 328-331 bp 1.0 81.1
petL-petG_p5 CCCAGCTCTGTTGATTGGTT CTGATCACCGCGTCTGTATT 50.0 50.0 58.1 57.8 329-332 bp 1.0 80.1