Hotspot

psbE-petL

LSC · 62983-64022 · 1040 bp

Back to Moraea

Pi 0.0321
MarkerSeek score 71.10
Primer available yes
Label psbE-petL

Feature Metadata

label_name
psbE-petL
feature_type
igs
region
LSC
length_bp
1040
variable_sites
33
pi
0.0321
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
71.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbE-petL_p1 CAACCCGCAATGAATAGGGA AACCAATCAACAGAGCTGGG 50.0 50.0 57.9 58.1 1217-1236 bp 1.0 81.8
psbE-petL_p2 CAACCCGCAATGAATAGGGA CAATCAACAGAGCTGGGGTT 50.0 50.0 57.9 58.1 1214-1233 bp 1.0 81.8
psbE-petL_p3 ACGAATAACCAACCCGCAAT AACCAATCAACAGAGCTGGG 45.0 50.0 58.2 58.1 1226-1245 bp 1.0 80.8
psbE-petL_p4 ACGAATAACCAACCCGCAAT CAATCAACAGAGCTGGGGTT 45.0 50.0 58.2 58.1 1223-1242 bp 1.0 80.8
psbE-petL_p5 CGAATAACCAACCCGCAATG CAATCAACAGAGCTGGGGTT 50.0 50.0 57.8 58.1 1222-1241 bp 1.0 80.7