Hotspot

petA-psbJ

LSC · 61386-62213 · 828 bp

Back to Moraea

Pi 0.0472
MarkerSeek score 67.50
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
828
variable_sites
18
pi
0.0472
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.46
markerseek_score
67.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 GGCATCTGTTATTTTGGCACA GCTGGTATTCCTGTGATCGG 42.9 55.0 57.4 58.1 564-988 bp 1.0 72.1
petA-psbJ_p2 TGGCATCTGTTATTTTGGCAC GCTGGTATTCCTGTGATCGG 42.9 55.0 57.4 58.1 565-989 bp 1.0 72.1
petA-psbJ_p3 GGCATCTGTTATTTTGGCACA GGTTCATATTCCGGGTTGGG 42.9 55.0 57.4 58.3 522-946 bp 1.0 70.7
petA-psbJ_p4 TGGCATCTGTTATTTTGGCAC GGTTCATATTCCGGGTTGGG 42.9 55.0 57.4 58.3 523-947 bp 1.0 70.7
petA-psbJ_p5 GGCATCTGTTATTTTGGCACA TTCCCCTTTGGCTGATAGGT 42.9 50.0 57.4 58.3 590-1014 bp 1.0 70.5