Hotspot

psaI-pafII

LSC · 58313-58722 · 410 bp

Back to Moraea

Pi 0.026
MarkerSeek score 68.00
Primer available yes
Label psaI-pafII

Feature Metadata

label_name
psaI-pafII
feature_type
igs
region
LSC
length_bp
410
variable_sites
10
pi
0.026
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.94
markerseek_score
68.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaI-pafII_p1 ATTTCCGGCAATTGCAATGG AAGAAGGATACAGGCCCAGA 45.0 50.0 58.0 57.7 536-558 bp 1.0 82.9
psaI-pafII_p2 ATTTCCGGCAATTGCAATGG AGAAGGATACAGGCCCAGAA 45.0 50.0 58.0 57.7 535-557 bp 1.0 82.9
psaI-pafII_p3 GGCAATTGCAATGGCTTCTT AAGAAGGATACAGGCCCAGA 45.0 50.0 57.9 57.7 530-552 bp 1.0 82.3
psaI-pafII_p4 GGCAATTGCAATGGCTTCTT AGAAGGATACAGGCCCAGAA 45.0 50.0 57.9 57.7 529-551 bp 1.0 82.3
psaI-pafII_p5 GGCCTAGTATTTCCGGCAAT AGAAGGATACAGGCCCAGAA 50.0 50.0 57.7 57.7 543-565 bp 1.0 80.9