Hotspot

rbcL-accD

LSC · 55615-56293 · 679 bp

Back to Moraea

Pi 0.0446
MarkerSeek score 72.80
Primer available yes
Label rbcL-accD

Feature Metadata

label_name
rbcL-accD
feature_type
igs
region
LSC
length_bp
679
variable_sites
30
pi
0.0446
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
72.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rbcL-accD_p1 ATTCGAGCCGGTAGATACGA GACAACATCAAATTGAACCACA 50.0 36.4 58.1 55.7 742-775 bp 1.0 48.9
rbcL-accD_p2 TTCGAGCCGGTAGATACGAT GACAACATCAAATTGAACCACA 50.0 36.4 58.1 55.7 741-774 bp 1.0 48.9
rbcL-accD_p3 CGCTGCTTGTGAAGTATGGA GACAACATCAAATTGAACCACA 50.0 36.4 58.3 55.7 778-811 bp 1.0 47.5
rbcL-accD_p4 ATTCGAGCCGGTAGATACGA ACAACATCAAATTGAACCACA 50.0 33.3 58.1 54.5 741-774 bp 1.0 46.8
rbcL-accD_p5 TTCGAGCCGGTAGATACGAT ACAACATCAAATTGAACCACA 50.0 33.3 58.1 54.5 740-773 bp 1.0 46.8