Hotspot

ycf3-trnS-GGA

LSC · 43780-44147 · 368 bp

Back to Moraea

Pi 0.0326
MarkerSeek score 61.30
Primer available yes
Label ycf3-trnS-GGA

Feature Metadata

label_name
ycf3-trnS-GGA
feature_type
igs
region
LSC
length_bp
368
variable_sites
12
pi
0.0326
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
61.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf3-trnS-GGA_p1 TGGCTACAATTGAAGAGGTCT GAACCACTCGGCCATCTTTC 42.9 55.0 56.6 58.9 447-455 bp 1.0 56.0
ycf3-trnS-GGA_p2 TGGCTACAATTGAAGAGGTCTT GAACCACTCGGCCATCTTTC 40.9 55.0 57.4 58.9 447-455 bp 1.0 53.7
ycf3-trnS-GGA_p3 TTGGCTACAATTGAAGAGGTCT GAACCACTCGGCCATCTTTC 40.9 55.0 57.4 58.9 448-456 bp 1.0 53.7
ycf3-trnS-GGA_p4 TGGCTACAATTGAAGAGGTCT AACCACTCGGCCATCTTTC 42.9 52.6 56.6 57.8 446-454 bp 1.0 53.3
ycf3-trnS-GGA_p5 TGGCTACAATTGAAGAGGTCT GAACCACTCGGCCATCTTT 42.9 52.6 56.6 57.8 447-455 bp 1.0 53.3