Hotspot

trnG-GCC-trnfM-CAU

LSC · 35818-36043 · 226 bp

Back to Moraea

Pi 0.0402
MarkerSeek score 68.80
Primer available yes
Label trnG-GCC-trnfM-CAU

Feature Metadata

label_name
trnG-GCC-trnfM-CAU
feature_type
igs
region
LSC
length_bp
226
variable_sites
9
pi
0.0402
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
68.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-GCC-trnfM-CAU_p1 AGAAGATGCGGGTTCGATTC GTTCAAATCCTGTCTCCGCA 50.0 50.0 58.1 58.2 279-286 bp 1.0 81.1
trnG-GCC-trnfM-CAU_p2 GAGAAGATGCGGGTTCGATT GTTCAAATCCTGTCTCCGCA 50.0 50.0 58.1 58.2 280-287 bp 1.0 81.1
trnG-GCC-trnfM-CAU_p3 AGAAGATGCGGGTTCGATTC GTAGCTCGCAAGGCTCATAA 50.0 50.0 58.1 57.8 313-320 bp 1.0 80.5
trnG-GCC-trnfM-CAU_p4 AGAAGATGCGGGTTCGATTC TAGCTCGCAAGGCTCATAAC 50.0 50.0 58.1 57.8 312-319 bp 1.0 80.5
trnG-GCC-trnfM-CAU_p5 GAGAAGATGCGGGTTCGATT TAGCTCGCAAGGCTCATAAC 50.0 50.0 58.1 57.8 313-320 bp 1.0 80.5