Hotspot

psbH

LSC · 78731-78952 · 222 bp

Back to Moquilea

Pi 0.0045

MarkerSeek score 40.30

Primer available yes

Label psbH

Feature Metadata

label_name: psbH
feature_type: gene
region: LSC
length_bp: 222
variable_sites: 1
pi: 0.0045
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbH_p1	TCAACTAGTCCCCGTGTTCT	CCGTGCTCCAAATACTCGAA	50.0	50.0	58.0	58.0	726 bp	1.0	80.3
psbH_p2	TCAACTAGTCCCCGTGTTCT	TTCCGTGCTCCAAATACTCG	50.0	50.0	58.0	58.0	728 bp	1.0	80.3
psbH_p3	TTCCTTCAACTAGTCCCCGT	CCGTGCTCCAAATACTCGAA	50.0	50.0	58.0	58.0	731 bp	1.0	80.1
psbH_p4	TTCCTTCAACTAGTCCCCGT	TTCCGTGCTCCAAATACTCG	50.0	50.0	58.0	58.0	733 bp	1.0	80.1
psbH_p5	GTGTTCTTCGAAGGGATCCC	CCGTGCTCCAAATACTCGAA	55.0	50.0	58.0	58.0	713 bp	1.0	80.1