Hotspot

ndhF

SSC · 115843-118131 · 2289 bp

Back to Moquilea

Pi 0.00619

MarkerSeek score 47.30

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2289
variable_sites: 14
pi: 0.00619
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 47.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	GCAGAATACCGTCACCCATT	GAGCCGATGAATCAGAACCA	50.0	50.0	58.0	57.7	2818-2878 bp	1.0	79.4
ndhF_p2	AAACGAAGGGGGCTAAACAG	GAGCCGATGAATCAGAACCA	50.0	50.0	58.1	57.7	2704-2764 bp	1.0	79.1
ndhF_p3	GAAACGAAGGGGGCTAAACA	GAGCCGATGAATCAGAACCA	50.0	50.0	58.1	57.7	2705-2765 bp	1.0	79.0
ndhF_p4	TGAAACGAAGGGGGCTAAAC	GAGCCGATGAATCAGAACCA	50.0	50.0	58.1	57.7	2706-2766 bp	1.0	79.0
ndhF_p5	CTGAAACGAAGGGGGCTAAA	GAGCCGATGAATCAGAACCA	50.0	50.0	57.8	57.7	2707-2767 bp	1.0	78.3