Hotspot

ndhG-ndhI

SSC · 125392-125851 · 460 bp

Back to Moquilea

Pi 0.0159

MarkerSeek score 68.90

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: SSC
length_bp: 460
variable_sites: 7
pi: 0.0159
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 68.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	TCCCAACGAAAAGGCAGAAT	TGCTTTAGGGCGTTTACCAA	45.0	45.0	57.7	57.7	658-677 bp	1.0	78.6
ndhG-ndhI_p2	TCCCAACGAAAAGGCAGAAT	TTGCTTTAGGGCGTTTACCA	45.0	45.0	57.7	57.7	659-678 bp	1.0	78.6
ndhG-ndhI_p3	TCCCAACGAAAAGGCAGAAT	AGGGCGTTTACCAATGTCAA	45.0	45.0	57.7	57.7	652-671 bp	1.0	78.6
ndhG-ndhI_p4	ATTGGTCCAGGCAAATCCAT	TGCTTTAGGGCGTTTACCAA	45.0	45.0	57.4	57.7	558-577 bp	1.0	77.0
ndhG-ndhI_p5	ATTGGTCCAGGCAAATCCAT	TTGCTTTAGGGCGTTTACCA	45.0	45.0	57.4	57.7	559-578 bp	1.0	77.0