Hotspot

psbT-psbN

LSC · 78406-78484 · 79 bp

Back to Moquilea

Pi 0.0759

MarkerSeek score 71.90

Primer available yes

Label psbT-psbN

Feature Metadata

label_name: psbT-psbN
feature_type: igs
region: LSC
length_bp: 79
variable_sites: 6
pi: 0.0759
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbT-psbN_p1	TCGAGAACCGCCTAAAGTTC	GGGATCCCTTCGAAGAACAC	50.0	55.0	57.6	58.0	148 bp	1.0	80.2
psbT-psbN_p2	TCGAGAACCGCCTAAAGTTC	AGGGATCCCTTCGAAGAACA	50.0	50.0	57.6	58.0	149 bp	1.0	80.0
psbT-psbN_p3	GAGAACCGCCTAAAGTTCCA	GGGATCCCTTCGAAGAACAC	50.0	55.0	57.5	58.0	146 bp	1.0	79.3
psbT-psbN_p4	GAGAACCGCCTAAAGTTCCA	AGGGATCCCTTCGAAGAACA	50.0	50.0	57.5	58.0	147 bp	1.0	79.1
psbT-psbN_p5	CGAGAACCGCCTAAAGTTCC	GGGATCCCTTCGAAGAACAC	55.0	55.0	58.6	58.0	147 bp	1.0	77.9