Hotspot

accD-psaI

LSC · 61656-62493 · 838 bp

Back to Moquilea

Pi 0.00494

MarkerSeek score 64.90

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 838
variable_sites: 4
pi: 0.00494
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 64.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	AAAGGGCGTTCTGAATGAGT	CCATTGCAATTGCCGGAAAT	45.0	45.0	57.4	58.0	1001-1002 bp	1.0	77.9
accD-psaI_p2	AAGGGCGTTCTGAATGAGTT	CCATTGCAATTGCCGGAAAT	45.0	45.0	57.4	58.0	1000-1001 bp	1.0	77.9
accD-psaI_p3	GCTCCACGCTTTCTTTCCTT	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.8	58.0	974-975 bp	1.0	76.4
accD-psaI_p4	AAAGGGCGTTCTGAATGAGT	GCCGGAAATACTAACCCCAC	45.0	55.0	57.4	58.3	990-991 bp	1.0	75.5
accD-psaI_p5	AAGGGCGTTCTGAATGAGTT	GCCGGAAATACTAACCCCAC	45.0	55.0	57.4	58.3	989-990 bp	1.0	75.5