Hotspot

atpB-rbcL

LSC · 57088-57902 · 815 bp

Back to Moquilea

Pi 0.00629

MarkerSeek score 64.70

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 815
variable_sites: 5
pi: 0.00629
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 64.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	GCCAATGATTTGAGCGATCC	GCCTTGAATCCAACACTTGC	50.0	50.0	57.6	57.9	923-943 bp	1.0	78.3
atpB-rbcL_p2	GGCCAATGATTTGAGCGATC	GCCTTGAATCCAACACTTGC	50.0	50.0	57.6	57.9	924-944 bp	1.0	78.3
atpB-rbcL_p3	GCCAATGATTTGAGCGATCC	CCAGCCTTGAATCCAACACT	50.0	50.0	57.6	58.1	926-946 bp	1.0	78.2
atpB-rbcL_p4	GGCCAATGATTTGAGCGATC	CCAGCCTTGAATCCAACACT	50.0	50.0	57.6	58.1	927-947 bp	1.0	78.2
atpB-rbcL_p5	TTTTCAAACGCGGAAACTCC	GCCTTGAATCCAACACTTGC	45.0	50.0	57.5	57.9	889-909 bp	1.0	77.8