Hotspot

ndhC-trnV-UAC

LSC · 52835-54000 · 1166 bp

Back to Moquilea

Pi 0.00787
MarkerSeek score 65.10
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1166
variable_sites
9
pi
0.00787
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
65.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 ATGGGGGATAAAACTCCGGA GCATGTTGGGTCTTTGAAGC 50.0 50.0 58.1 57.9 1353-1364 bp 1.0 80.5
ndhC-trnV-UAC_p2 CTGGACCTTTGGTAATGGGG GCATGTTGGGTCTTTGAAGC 55.0 50.0 57.9 57.9 1367-1378 bp 1.0 80.1
ndhC-trnV-UAC_p3 TCCGGAAATTCGAAATGCCA GCATGTTGGGTCTTTGAAGC 45.0 50.0 58.2 57.9 1339-1350 bp 1.0 79.9
ndhC-trnV-UAC_p4 ATGGGGGATAAAACTCCGGA AGAAGGTCTACGGTTCGAGT 50.0 50.0 58.1 57.8 1289-1300 bp 1.0 79.6
ndhC-trnV-UAC_p5 CTGGACCTTTGGTAATGGGG AGAAGGTCTACGGTTCGAGT 55.0 50.0 57.9 57.8 1303-1314 bp 1.0 79.2