Hotspot

rps2-rpoC2

LSC · 16556-16806 · 251 bp

Back to Moquilea

Pi 0.0159

MarkerSeek score 48.80

Primer available yes

Label rps2-rpoC2

Feature Metadata

label_name: rps2-rpoC2
feature_type: igs
region: LSC
length_bp: 251
variable_sites: 4
pi: 0.0159
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps2-rpoC2_p1	AATGAACTCCTGCTTCCAGC	ATGATCCAGCAGAACAATCA	50.0	40.0	58.2	54.7	336-353 bp	1.0	53.3
rps2-rpoC2_p2	AATGAACTCCTGCTTCCAGC	TGATCCAGCAGAACAATCAT	50.0	40.0	58.2	54.7	335-352 bp	1.0	53.3
rps2-rpoC2_p3	AATGAACTCCTGCTTCCAGC	TGATCCAGCAGAACAATCATT	50.0	38.1	58.2	55.6	335-352 bp	1.0	52.0
rps2-rpoC2_p4	AATGAACTCCTGCTTCCAGC	ATGATCCAGCAGAACAATCAT	50.0	38.1	58.2	55.3	336-353 bp	1.0	50.2
rps2-rpoC2_p5	AATGAACTCCTGCTTCCAGC	TGATCCAGCAGAACAATCATTT	50.0	36.4	58.2	56.3	335-352 bp	1.0	50.1