Hotspot

atpA-atpF

LSC · 11489-11552 · 64 bp

Back to Moquilea

Pi 0.0781

MarkerSeek score 84.80

Primer available yes

Label atpA-atpF

Feature Metadata

label_name: atpA-atpF
feature_type: igs
region: LSC
length_bp: 64
variable_sites: 5
pi: 0.0781
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 84.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA-atpF_p1	TACTAATCTCGTCGGCTCGA	TATTGGCATGTTTGGAGCGA	50.0	45.0	57.8	57.9	135 bp	1.0	80.2
atpA-atpF_p2	CCGTCGCCTACTTGAAGTAC	TATTGGCATGTTTGGAGCGA	55.0	45.0	58.1	57.9	217 bp	1.0	80.1
atpA-atpF_p3	GTCGCCTACTTGAAGTACGG	TATTGGCATGTTTGGAGCGA	55.0	45.0	58.1	57.9	215 bp	1.0	80.1
atpA-atpF_p4	TAATCTCGTCGGCTCGAATG	TATTGGCATGTTTGGAGCGA	50.0	45.0	57.6	57.9	132 bp	1.0	78.9
atpA-atpF_p5	ACTTGAAGTACGGTACCGGT	TATTGGCATGTTTGGAGCGA	50.0	45.0	58.4	57.9	208 bp	1.0	77.9